I love to travel so of course this blog will be mostly about my travel. I also love to eat! So there will also be some entries about my search for food. I am Mon Zaffarin and this is
The process according to claim 1, wherein the steps a to f are performed in the order as indicated in claim 1. The process according to any one of claims 1 to 5, wherein the water-miscible organic solvent Mr jasa andrensek selected from C1-C4 alcohols, C3-C4 ketones or C2-C4 nitriles.
The process according to any one of claims 1 to 6, wherein the step of desorbing teicoplanin from the hydrophobic adsorption resin is performed using an aqueous medium having a pH value below 4.
The process according to claim 7, wherein the pH value of the aqueous medium is adjusted by addition of an aqueous solution of a mineral acid, an organic acid or an acidic buffer. The process according to any one of claims 1 to 12, wherein obtaining purified teicoplanin in step f is performed by teicoplanin isolation involving removal of the solvent using evaporation, vacuum evaporation, lyophilisation, spray drying or precipitation by adding antisolvent.
A process for manufacturing a pharmaceutical composition comprising the process according to any one of claims 1 to 13, and wherein purified teicoplanin is admixed with at least one pharmaceutical excipient or carrier. Field of the Invention The present invention relates to the field of chemical technology, more specifically to a novel and effective way Mr jasa andrensek purification of the antibiotic teicoplanin.
Background of the invention Teicoplanin is a commonly used term for a group of glycopeptide antibiotics from the vancomycin-ristocetin family, produced by a fungal microorganism such as e. Teicoplanin is firstly disclosed in J.
Antibiotics 31p. Teicoplanin A2 was later recognized as a mixture of five molecules J. Antibiotics 37p. An exemplified and typical compound representation of teicoplanin is illustrated in Fig.
These five A2 compounds Mr jasa andrensek teicoplanin together with another major component A3 and some minor components e. Besides vancomycin and vancomycin-like antibiotics it still remains a key active compound against highly resistent microorganisms. A number of efforts have been made to separate teicoplanin from a culture broth of the microorganism and to purify teicoplanin to reach pharmaceutical grade.
According to the first publication in USa culture broth is divided into a mycelial cake and a filtrate. After treatment of the cake both fractions were extracted with butanol at acidic pH and the crude fermentation product was precipitated after partial removal of the solvent.
However, this process is a laboratory trial of isolation with several disadvantages for industrial use in view of complexity, quality, costs and yields. In order to improve yields of fermentation in such self-inhibited production of teicoplanin the product was tried to be continuously removed by adding water-miscible organic solvents, such as acetone, n-propanol, and acetonitrile Korean Pat.
But such processes still need large volumes of organic solvents to extract teicoplanin causing handling problems and environmental pollution. US patent application 4, reports a process for purification of teicoplanin involving a cation exchange technique without prior pH adjustment.
Teicoplanin is eluted from the cation exchange resin using a solution having a pH value of from about 9 to about Further progress in the isolation of teicoplanin was made by discovery of the efficient binding of teicoplanin to hydrophobic resins, firstly disclosed in EPusing a special polyamide resin and further upgrading the purification by an extraction process of teicoplanin from mycelium at alkaline pH as a simplification of primary disclosed processes EP The introduction of commercial hydrophobic resins, preferably applied as a column rather than in suspension treatment, represented a further progress.
However, the approach required the continuous neutralization of the eluting solution in order to prevent epimerisation of the alkali-sensitive product. However, in case that the filtered culture broth extracted from a basic solution is adsorbed to a resin, such as Diaion HP 20, a substantial amount of teicoplanin is lost in the adsorption step, and the purity of teicoplanin eluted by a methanol concentration gradient is very low.
Moreover, it is necessary to remove methanol in order to apply the solution to a lectin-immobilized resin. Furthermore, it is not desirable to apply the strategy in large scale production because of the cost of lectin-immobilized resins.
According to Korean pat.
The eluting solution containing teicoplanin is concentrated by vacuum distillation, treated with an activated carbon, and subjected to a precipitation process, and thereby teicoplanin is purified.
Again, the process is insufficient for a scale up due to the irreversible adsorption of teicoplanin to the resin reducing the yield and due to the use of large amounts of solvent.
Alternatively, some other studies have included reverse phase resins to separate and purify teicoplanin from the culture broth. For example, a process, published in Chromatographia 24 p. Two other alternatives accomplished the chromatographic methods on reverse phase resins by additional manners of purification.
For example, Korean pat. US recites a process of purifying teicoplanin from a culture broth, which includes a primary purifying step using a synthetic adsorbent, a secondary purifying using a cation-exchange resin, a catalytic resin, or a chelate resin, a tertiary purifying step using a reverse phase resin, and a final lyophilisation step.
Accordingly, the use of more expensive reverse phase alone cannot avoid the use of additional adsorbents, so it does not justify the additional costs. In summary, all above described methods offer only partial solutions, which do not solve the complexity of isolation and purification of teicoplanin.
It went back to the adsorption method on hydrophobic resins describing the following key steps of purification and isolation of teicoplanin: Although this process may represent the best literature option of isolation of teicoplanin, its reproduction surprisingly shows considerable drawbacks: A later publication CN A represented a revision of said comprehensive procedure by adjusting teicoplanin solution to 2,5 to 6 following the previous steps such as: Benefits of methods of these previous steps are not clearly identified.
The ultrafiltration as an alternative to the nanofiltration is especially unclear, because the molecular mass of teicoplanin lies between average cut-offs of nanomembranes and ultramembranes, so their function should not be the same.
Hence, there is still a need for an efficient comprehensive procedure of isolation of teicoplanin of pharmaceutical grade.MANHATTAN, NEW YORK, APRIL 28, The Consul General of Malaysia, Mr. Jeremiah Oliver Jitos and the home based staff of the Consulate, attended the ASEAN Short Film Festival Read More» Anugerah Pingat Jasa Malaysia (PJM).
Oct 07, · The table shows that a skilled person may collect fractions taking into consideration a balance between economy of the process and technical simplicity on one side and regulatory demands including safety for patients on the other side.
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Nov 22, · Mr. Mon's Journey. Hello all! is the year I started this blog to share about my passion, hobby and experiences I love to travel so of course this blog will be mostly about my travel. pfmlures.com Be a member of a first class finance community. Join finexpert as a individual member and enjoy the unique benefits of your membership: weekly newsletter, overview finance studies and research papers, notes, video lectures, search function.
Former Director of JASA House, a facility for women of domestic violence • Literacy Advance • Escape Center: Mr. Ryland, President of the Texas Tuck Alumni Executive Management program, is a graduate of Dartmouth and the Kellogg School of Business, Executive Business Program.